The effect of temperature on the enzyme activity of peroxidase

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The effect of temperature on the enzyme activity of peroxidase

Peer reviewers approved by Prof. Yongzhong Wang Peer reviewer comments 4 Editor who approved publication: In recent years, food storage environment safety has been a major concern for food and health scientists.

There is growing interest in electrochemical biosensors due to their high sensitivity and rapid response. The aim of this review article is to provide details regarding the development of enzyme-based electrochemical biosensors, and their use in the detection of a range of chemical and biological compounds in the food industry.

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We have focused on the basic principle, generation, classification, and application of electrochemical biosensors for food safety. Microorganisms such as pathogens, viruses, and bacterium are present in food, and partially or completely destroy the nutritional quality of food.

Food-borne pathogens are a major threat to food safety. These methods are more time consuming, laborious, and require a well-trained person. There is an increasing demand for developing a fast and sensitive technique to monitor food-borne pathogens. A biosensor is a device or instrument that comprises a biological sensing material combined with a chemical or physical transducer, which converts a chemical or biological signal into an electrical signal.

Figure 1 A typical representation of a biosensor. Biosensors have many applications in food pathogen, additivesenvironmental monitoring toxic pollutionclinical diagnoses glucose in blood, cholesteroland biodefense biowarfare due to their selectivity, sensitivity, stability, and quick response time.

This review provides information regarding the history, classification, and application of electrochemical biosensors for food safety. Historical perspective The first glucose sensor was proposed by Clark and Lyon 6 from Cincinnati Hospital in An oxygen electrode is entrapped in a thin layer of glucose oxidase GOx via a semipermeable dialysis membrane in which the oxygen consumption is monitored by the enzyme-catalyzed reaction.

InGuilbault and Montalvo reported the first enzyme-based potentiometric biosensor in which urease enzyme was immobilized on an ammonia electrode for the detection of urea.

InGuilbault and Lubrano developed an amperometric enzyme electrode for the determination of glucose in blood samples and monitored the amount of liberated hydrogen peroxide.

Key Points

When voltage is applied, the oxidation of H2O2 takes place, which results in an electric signal. The active site and the FAD prosthetic group are buried deep within the enzyme, which restricts the diffusion of reagents, 11 and second, there is limited solubility of O2 in aqueous media.

Figure 2 Diagrammatic representation of the first-generation of a glucose biosensor. FAD, flavin adenine dinucleotide.

Second-generation glucose biosensor The first-generation biosensor needs further improvement due to the oxygen deficiency. The limitation of the first-generation glucose biosensor is removed by replacing the oxygen with redox electron mediators that carry electrons from enzyme to electrode.

The diagrammatic representation of the second-generation glucose biosensor is shown in Figure 3. The second-generation biosensor has the defect of leaching the mediator.

Figure 3 Diagrammatic representation of the second-generation of a glucose biosensor.Key Points. Honey is a traditional topical treatment for infected wounds. It can be effective on antibiotic-resistant strains of bacteria.

Honey is produced from many different floral sources and its antibacterial activity varies with origin and processing.

Cofactors. The catalytic activity of many enzymes depends on the presence of small molecules termed cofactors, although the precise role varies with the cofactor and the attheheels.com an enzyme without its cofactor is referred to as an apoenzyme; the complete, catalytically active enzyme is called a holoenzyme..

Cofactors can be subdivided into two groups: metals and small organic molecules. It was found that the enzymatic activity was optimum at 37 degree Celsius and the enzyme activity decreased above that temperature thus nullifying the hypothesis that the reaction rate increases as temperature increases based on Arrhenius equation.

Peroxidase and polyphenoloxidase activities of litchi pericarp Sci. Agric. (Piracicaba, Braz.), v, n.2, p, March/April Effect of pH and temperature on peroxidase and.

The effect of temperature on the enzyme activity of peroxidase

Enzyme Action: Testing Catalase Activity (O 2) Advanced Biology with Vernier 2 - 3 (O 2) Figure 1 5. When data collection has finished, remove the O 2 gas sensor from the Nalgene bottle.

Rinse the bottle with water and dry with a paper towel. Enzyme-based electrochemical biosensors for food safety: a review Harish Kumar, Rani Neelam Electrochemistry Laboratory, Department of Chemistry, Chaudhary Devi Lal University, Sirsa, Haryana, India Abstract: In recent years, food storage environment safety has been a major concern for food and health scientists.

There is growing interest in electrochemical biosensors due to their high.

[Full text] Enzyme-based electrochemical biosensors for food safety: a review | NDD